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Definitions from the Web

Stationary Phase

Description:

The term "stationary phase" has different meanings depending on the context. It can refer to:

1. In chromatography, the stationary phase is the immobile component of the technique used to separate and identify chemical compounds.
2. In microbiology, the stationary phase is a stage in microbial growth where the number of viable cells remains relatively constant due to depletion of nutrients or accumulation of inhibitory substances.

Chromatography:

In chromatography, the stationary phase constitutes the fixed material through which the mobile phase flows. It could be a solid adsorbent or a liquid coating on a solid support. The stationary phase interacts differently with each component of the sample, facilitating their separation.

Example Sentences:

• The polar stationary phase in this chromatographic column allows for the separation of the polar compounds in the mixture.
• When the mobile and stationary phases are well-balanced, chromatographic separations can be highly efficient.
• Scientists often modify the stationary phase to enhance selectivity and improve separation performance.

Microbiology:

In microbiology, the stationary phase represents the stage in the growth of microorganisms where cell division arrests due to environmental changes within the culture medium. Nutrient depletion and accumulation of toxic metabolites contribute to this phase.

Example Sentences:

• The bacteria reached the stationary phase after 48 hours of growth, with no further increase in cell numbers.
• During the stationary phase, microbial cells undergo physiological changes to enhance survival under stressful conditions.
• Microbes in the stationary phase might exhibit increased antibiotic tolerance compared to those in the exponential growth phase.

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