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Definitions from the Web

Biotinylated Probe

A biotinylated probe refers to a molecule or a fragment of DNA or RNA that has been labeled with biotin, a vitamin B7 derivative, for various scientific applications. The biotinylated probe is used to detect and bind to specific target molecules in biological samples, enabling the identification, visualization, and analysis of these molecules.

Senses and Usages:

1. Molecular Biology:

In molecular biology, a biotinylated probe can be used for:

• Hybridization: Biotinylated probes allow the specific binding to complementary nucleic acid sequences, facilitating the detection of DNA or RNA of interest.
• Fluorescence In Situ Hybridization (FISH): Biotinylated probes labeled with fluorophores enable the visualization of specific DNA sequences within cells or tissues.
• Chromatin Immunoprecipitation (ChIP): Biotinylated probes aid in the isolation and enrichment of DNA fragments associated with specific proteins.

2. Biochemistry:

In biochemistry, a biotinylated probe can be utilized for:

• Protein Detection: Biotinylated probes can be used in techniques such as Western blotting or enzyme-linked immunosorbent assay (ELISA) to detect and quantify specific proteins.
• Protein Interaction Studies: Biotinylated probes can be employed to study protein-protein interactions or protein-ligand interactions.

Sample Sentences:

1. The researchers used a biotinylated probe to identify the specific gene sequences responsible for the observed trait.

2. The biotinylated probe successfully detected the presence of the target protein in the experimental sample.

3. By employing a biotinylated probe, the scientists were able to visualize the localization of the mRNA molecules within the cells.

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